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You are watching: Why did hershey and chase use the isotopes 32p and 35s in their experiments?

What crucial discovery did Griffith make in his pneumonia experiments on mice?
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He discovered that there was a revolution in which one strain of bacteria (a harmless strain) was somejust how changed permanently into one more (the illness causing strain). He hypothesized that the transforming aspect can be a gene bereason the ability to reason illness ws inherited by the transformed bacteria's offspring.
Exactly what did Griffith carry out to the mice in the experiment wright here he learned around transformation?He injected mice with his heat-eliminated, disease-causing bacteria (the heated ones that killed the bacteria) with live, harmmuch less ones. (check out 288) So, the harmless bacteria had actually been transcreated into disease-bring about genes.
What did Aexceptionally and also his colleagues identify caused the transformation of harmless bacteria right into illness leading to bacteria?They discovered that nucleic acid DNA stores and also transmits the hereditary information from one generation of an organism to the next.
What is a bacteriophage?It is a virus that infects and kills bacteria. (It indicates "bacteria eater".)
Of what are bacteriophage made?They're made of a DNA or RNA core and also a protein coat.
What happens as soon as a bacteriophage enters a bacterium?It attaches to the surchallenge of the cell and inject its viral genetic indevelopment into the cell. These viral genes create many type of new bacteriophages that progressively damage the bacterium. When that cell splits, numerous new virprovides are released.
What were Hershey and also Chase trying to determine in their experiments?They wanted to understand whether the protein coat or the DNA core of the virus gone into the infected cell; this would help them determine whether genes were made up protein or of DNA.
What technique did Hershey and Chase usage to examine the viruses in their experiments?They provided radioenergetic isotopes of phosphorus-32 and also sulfur-35 as markers so they could map the proteins and DNA to see what they were doing to the cell.
Why were phosphorus-32 and also sulfur-35 perfect for what Hershey & Chase were investigating in their experiments?They were perfect bereason proteins contain almost no phosphorus and DNA consists of no sulhair, so the radioenergetic isotopes would certainly show up in the cells if the phosphorus-32 or sulfur-35 showed up. If sulfur-35 showed up in the bacteria, it intended the viruses' protein had been injected into the bacteria; if the phosphorus-32 was discovered in the bacteria, the DNA had actually been injected right into the bacteria.
What conclusion did Hershey & Chase make regarding the hereditary product that was in bacteriophage?They concluded that the genetic material of the bacteriophage was DNA, NOT protein.
What 3 points perform genes do that interested scientists?1) genes had to carry information from 1 generation to the next; 2) they put that indevelopment to occupational by determining the heritable characteristics of organisms; 3) genes had actually to be quickly replicated because every one of a cell's genetic information is replicated eincredibly time a cell divides.
What are nucleotides?They are the monomers of nucleic acids comprised of a 5-carbon sugar, a phosphate group, and also a nitrogenous base (see 291).
What are the 4 kinds of nitrogenous bases in DNA?1) adenine & 2) guanine (both are purines); 3) cytosine and also 4) thymine (pyrimidines).
What is the substantial distinction between the structure of purines and pyrimidines?Purines have 2 rings in their structure (pg. 291); pyrimidines have actually one ring (photo on 291).
What is the "backbone" of a DNA chain?Sugar and phosphate are the backbone of a DNA chain.
What carry out Chargaff's Rules say?In any kind of sample of DNA, the percenteras of guanine (G) & cytosine (C) bases were almost equal; the exact same was true of the percentages of adenine (A) and thymine (T). So, = and also =
What approach did Rosalind Franklin usage in her examine of DNA?She supplied X-ray diffraction, aiming an effective X-ray beam at concentrated DNA samples and recorded the scattering pattern of the S-rays on film.
What vital hints re: DNA developed from Franklin's work?1) The X-shaped pattern shows that the strands in DNA are twisted about each various other in a shape known as a helix; 2) the angle of the X said tright here are 2 strands in the structure; 3) the nitrogenous baes are close to the facility of molecule.
What breakthrough did Watson & Crick make?They made a design of DNA reflecting a dual helix, in which 2 strands were wound roughly each other. They additionally uncovered that hydrogen bonds could create between particular nitrogenous bases and also administer sufficient pressure to organize the 2 strands together.

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What is "base pairing"?Watboy & Crick uncovered that For eincredibly adenine in a double-stranded DNA molecule, there had actually to be exacly one thymine molecule; for each cytosine molecule, there was one guanine molecule.
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